Literature summary for 3.4.21.106 extracted from

Moran, P.; Li, W.; Fan, B.; Vij, R.; Eigenbrot, C.; Kirchhofer, D.
Pro-urokinase-type plasminogen activator is a substrate for hepsin (2006), J. Biol. Chem., 281, 30439-30446.

Search for more literature for 3.4.21.106

Application

Application	Comment	Organism
medicine	hepsin may initiate plasmin-mediated proteolytic pathways at the tumor/stroma interface that lead to basement membrane disruption and tumor progression	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
inserted in a baculovirus expression vector under the control of a polyhedrin promoter and expressed in T.in.Pro cells. cDNA of full-length hepsin inserted into a mammalian expression vector overexpressed in LNCaP cells	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
HAI-2	is a potent inhibitor	Homo sapiens
HI-10331	reversible active site inhibitor	Homo sapiens
KD1	HAI-1B-derived Kunitz domain inhibitor	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0341	-	pro-urokinase-type plasminogen activator	-	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cell surface	-	Homo sapiens	9986	-

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
by nickel-nitrilotriacetic acid affinity chromatography, ion-exchange chromatography or affinity chromatography	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	present in LNCaP-17 and LNCaP-34 cells, absent in HPAC cells	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	does not cleave pro-tissue-type plasminogen activator	Homo sapiens	?	-	?
pro-urokinase-type plasminogen activator + H2O	cleavage at the Lys158-Ile159 (P1-P1') peptide bond	Homo sapiens	active high molecular weight urokinase-type plasminogen activator	-	?

Synonyms

Synonyms	Comment	Organism
type II transmembrane serine protease	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.00145	-	pro-urokinase-type plasminogen activator	-	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.00026	-	HAI-2	-	Homo sapiens
0.0416	-	HI-10331	-	Homo sapiens

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Release 2023.1 (January 2023)